Open AccessStreptococcus pyogenes grown under sublethal concentrations of penicillin G accumulates close to the septum pentapeptide subunits of peptidoglycan
Author(s) -
Seidl Peter H.,
Zwerenz Peter,
Golecki Jochen R.,
Schleifer Karl Heinz
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01104.x
Subject(s) - peptidoglycan , pentapeptide repeat , immunogen , microbiology and biotechnology , penicillin , streptococcus pyogenes , biology , streptococcus , antibody , cell wall , antibiotics , bacteria , biochemistry , immunology , monoclonal antibody , staphylococcus aureus , genetics , peptide
The accumulation of non‐crosslinked pentapeptide subunits of peptidoglycan was localized close to the septum in cells of Streptococcus pyogenes , grown under sublethal concentrations of penicillin G. Pentapeptide subunits were detected in the cell wall by the indirect immunoferritin technique, employing monospecific antibodies to the sequence R‐ d ‐Ala‐ d ‐Ala‐OH. Antibodies of this specificity were obtained by immunization with synthetic immunogen albumin‐(CH 2 CO‐Gly‐ l ‐Ala 2 ‐ d ‐Ala‐ d ‐Ala‐OH) 39 .