Open AccessConstruction of a cosmid library of Spirulina platensis as an approach to DNA physical mapping
Author(s) -
Rossi Edda,
Riccardi Giovanna,
Sanangelantoni Anna Maria,
Ciferri Orio
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01019.x
Subject(s) - cosmid , spirulina (dietary supplement) , biology , heterologous , gene , genomic library , protein subunit , chloroplast dna , microbiology and biotechnology , biochemistry , genetics , chloroplast , peptide sequence , raw material , ecology
A Spirulina platensis gene library has been constructed using cosmid vector pMMB34. The cosmid bank was controlled for its random gene distribution by colony hybridization. Genes were identified using either homologous or heterologous probes of genes involved in photosynthesis (large and small subunit of d ‐ribulose 1,5‐bisphosphate carboxylase, 32 kDa thylakoid protein, α, β subunits of C‐phycocyanin) and protein synthesis (elongation factors EF‐Tu, EF‐G).