Open AccessThe use of indole as a spectrophotometric assay substrate for toluene dioxygenase
Author(s) -
Jenkins Richard O.,
Dalton Howard
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01017.x
Subject(s) - toluene , pseudomonas putida , chemistry , absorbance , substrate (aquarium) , indole test , dioxygenase , dehydrogenase , chromatography , organic chemistry , enzyme , biology , ecology
Cell extracts of toluene‐grown Pseudomonas putida produced a soluble yellow dye during aerobic incubations with indole and NADH. Accumulation of indoxyl in reaction mixtures corresponded with a linear increase in absorbance at 400 nm. The rate of increase in absorbance was shown to be a specific measure of toluene dioxygenase activity. The primary product of toluene oxidation, cis ‐toluene dihydrodiol, inhibited dioxygenase activity in cell extracts containing no detectable activity of cis ‐toluene dihydrodiol dehydrogenase.