Open AccessCloning and expression of a Bacillus licheniformis α‐amylase gene in Pseudomonas aeruginosa
Author(s) -
Filloux A.,
Joyet P.,
Murgier M.,
Lazdunski A.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01012.x
Subject(s) - periplasmic space , bacillus licheniformis , cloning (programming) , amylase , extracellular , gene , enzyme , bacteria , molecular cloning , pseudomonas aeruginosa , chemistry , gene expression , biology , biochemistry , microbiology and biotechnology , escherichia coli , genetics , bacillus subtilis , computer science , programming language
The gene for B. licheniformis α‐amylase has been cloned in P. aeruginosa . Synthesis of the enzyme occurs in late log phase and goes on during stationary phase. Although P. aeruginosa is a secretory bacterium, α‐amylase is not efficiently secreted into the extracellular medium; 85% of the enzyme is retained in the periplasm.