Open AccessDenitrification by Rhizobium fredii *
Author(s) -
Hynes R.K.,
Ding A.L.,
Nelson L.M.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01008.x
Subject(s) - rhizobium , denitrification , microbiology and biotechnology , chemistry , bacteria , environmental science , biology , nitrogen , genetics , organic chemistry
Denitrification and anaerobic growth were investigated in fast‐growing isolates of Rhizobium fredii and in slow‐growing isolates of Bradyrhizobium japonicum . All isolates of R. fredii grew anaerobically, reducing NO − 3 (5.5 mM) to N 2 O in the absence and presence of C 2 H 2 . The stationary phase of growth was reached by most fast‐growing isolates after 50 h incubation, at which time NO − 3 was no longer detectable in the culture filtrate. Most slow‐growing isolates of B. japonicum , incubated under anaerobic conditions with NO − 3 , took twice as long to reach the stationary phase of growth as R. fredii . The terminal product of NO − 3 reduction in the slow‐growing isolates was either N 2 O or N 2 .