Open AccessTrehalose, a cytoplasmic reserve disaccharide of Cellulomonas sp. DSM20108: Its identification, carbon‐source‐dependent accumulation, and degradation during starvation
Author(s) -
Schimz KarlLudwig,
Irrgang Klaus,
Overhoff Barbara
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01005.x
Subject(s) - trehalose , disaccharide , chemistry , biochemistry , bacteria , carbon source , energy source , carbohydrate , carbon fibers , food science , microbiology and biotechnology , biology , organic chemistry , coal , genetics , materials science , composite number , composite material
During growth on complete medium, as well as on mineral salts medium with different carbon sources, Cellulomonas sp. synthesized trehalose (α‐ d ‐glucopyranosyl‐α‐ d ‐glucopyranoside). This non‐reducing disaccharide was accumulated intracellularly. Its specific concentration (up to 3 μmol per mg crude extract protein) reached a maximum during cultivation and was shown to be dependent on the carbon source used. During starvation at 30°C, trehalose was degraded in vivo at an average initial specific rate of about 0.5 μmol per mg crude extract protein per day. To our knowledge this is the first time that trehalose has been reported to be an energy storage compound in a prokaryotic microorganism.