Open AccessStreptomycin biosynthesis in Streptomyces griseus II. Adjacent genomic location of biosynthetic genes and one of two streptomycin resistance genes
Author(s) -
Distler J.,
Mansouri K.,
Piepersberg W.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01002.x
Subject(s) - streptomyces griseus , streptomycin , biology , gene , genetics , genomic dna , streptomyces coelicolor , dna , mutant , streptomyces , bacteria , antibiotics
Streptomyces griseus genomic DNA codes for two different streptomycin phosphotransferases. Both genes confer streptomycin resistance to their host. A cloned DNA fragment containing one of the two resistance determinants overlaps with another cloned segment of S. griseus genomic DNA, complementing 1 out of 3 classes of streptomycin‐idiotrophic mutants. This demonstrates physical linkage of resistance and biosynthetic genes involved in streptomycin biogenesis in the genome of S. griseus .