Open AccessStreptomycin biosynthesis in Streptomyces griseus I. Characterization of streptomycin‐idiotrophic mutants
Author(s) -
Distler J.,
Klier K.,
Piendl W.,
Werbitzky O.,
Böck A.,
Kresze G.,
Piepersberg W.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01001.x
Subject(s) - streptomyces griseus , streptomycin , biosynthesis , mutant , aminoglycoside , transamination , streptomycetaceae , streptomyces , biology , microbiology and biotechnology , actinomycetales , antibiotics , biochemistry , chemistry , bacteria , genetics , amino acid , enzyme , gene
A total of 16 idiotrophic mutants unable to produce the aminoglycoside antibiotic streptomycin ( smi ) were isolated from Streptomyces griseus N2‐3‐11. Cosynthesis of streptomycin, its formation from various precursors and analysis of accumulated intermediates allowed grouping of the mutants in 3 classes, blocked: (I) in the first transamination step of the streptidine pathway; (II) in later steps of the streptidine pathway; or (III) outside streptidine biosynthesis.