Open AccessCloning and expression of the Listeria monocytogenes haemolysin in Escherichia coli
Author(s) -
Vicente M.F.,
Baquero F.,
PérezDíaz J.C.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00988.x
Subject(s) - listeria monocytogenes , cosmid , hemolysin , biology , listeria , escherichia coli , microbiology and biotechnology , cloning (programming) , listeriolysin o , molecular cloning , genetics , dna , gene , bacteria , gene expression , virulence , computer science , programming language
Listeriolysin, an SH‐activated haemolysin probably involved in Listeria pathogenicity, has been cloned into the cosmid vector pHC79 and was expressed in Escherichia coli HB101 cells. Chromosomal DNA of Listeria monocytogenes serovar 1/2 was partially digested with Mbo I and ligated to the Bam HI cleaved cosmid. From 2000 recombinant clones examined, 12 (0.6%) produced haemolysin in solid and liquid media. All of them contained chromosome fragments of Listeria of about 40 kb. The cloning of the listeriolysin determinant will lead to a better understanding of the basis of Listeria pathogenicity.