Open AccessCharacterization of hup ‐specific DNA in Rhizobium leguminosarum strains of different origin *
Author(s) -
Nelson Louise M.,
Grosskopf E.,
Tichy H.V.,
Lotz W.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00984.x
Subject(s) - biology , rhizobium leguminosarum , rhizobiaceae , rhizobium , dna , hybridization probe , strain (injury) , gene , microbiology and biotechnology , bacteria , genetics , symbiosis , anatomy
17 Strains of Rhizobium leguminosarum which varied in phenotypic expression of uptake hydrogenase (Hup) were analyzed for the presence of hup ‐specific DNA sequences using hybridization probes constructed from hup ‐specific DNA cloned from Rhizobium japonicum (PHVT100) and R. leguminosarum (PHVT109 and PHVT115). 2 Hup − strains and 3 strains with trace amounts of Hup activity showed no hybridization. 10 Hup + strains hybridized with all 3 probes, indicating that hup ‐specific DNA sequences are conserved in these strains. 2 Hup + strains did not hybridize with any of the probes. These strains may possess significantly different DNA sequences determining the Hup phenotype.