Open AccessConvenient construction of strains useful for transducing recA mutations with bacteriophage P1
Author(s) -
Ihara Makoto,
Oda Yoshimitsu,
Yamamoto Kazuo
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00980.x
Subject(s) - tn10 , bacteriophage , escherichia coli , plasmid , biology , genetics , pbr322 , tetracycline , allele , microbiology and biotechnology , dna , enterobacteriaceae , gene , antibiotics
The generalized transducing phage P1 grew well on heterozygous Escherichia coli carrying recA srlC 300::Tn 10 on the chromosome and recA + on a pBR322‐derived plasmid. Because of the close linkage of Tn 10 to recA mutations, including recA 1, recA 13, recA 56, recA deletion and recA allele of E. coli BNG30, the latter can be moved to other strains in transductional crosses for selective resistance to tetracycline.