Open AccessPhosphorylation of phospholipase B in the plasma membrane of Saccharomyces cerevisiae
Author(s) -
Witt W.,
Wille P.,
Fuhrmann G.F.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00979.x
Subject(s) - immunoprecipitation , membrane , polyacrylamide gel electrophoresis , saccharomyces cerevisiae , biochemistry , phosphorylation , chemistry , vesicle , phospholipase , membrane protein , polyacrylamide , gel electrophoresis , enzyme , chromatography , yeast , gene , polymer chemistry
Plasma membrane vesicles from Saccharomyces cerevisiae were incubated with [γ‐ 32 P]ATP. Several phosphorylated protein bands were separated by LiDS polyacrylamide gel electrophoresis. One of these bands with an apparent M r of 145 000 was identified by immunoprecipitation as a membrane‐bound phospholipase.