Open AccessGM1 ELISA method for demonstration of Escherichia coli heat‐stable enterotoxin
Author(s) -
Svennerholm AnnMari,
Lindblad Marianne
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00974.x
Subject(s) - enterotoxin , escherichia coli , heat stable enterotoxin , heat labile enterotoxin , chemistry , cholera toxin , radioimmunoassay , microbiology and biotechnology , reagent , antibody , conjugate , chromatography , biology , biochemistry , immunology , gene , mathematical analysis , mathematics
A GM1‐ELISA for detection of the methanol soluble, heat‐stable enterotoxin (ST a ) produced by many enterotoxinogenic E. coli strains has been developed. This ST‐GM1‐ELISA, which is based on inhibition of binding of anti‐ST antibody to GM1‐bound ST‐cholera B subunit conjugates, is relatively simple and possible to perform with stable reagents and without any complicated equipment. By this method ST a could be detected in culture filtrates of human E. coli isolates with 100% sensitivity and specificity. The sensitivity of the method for purified ST is considerably higher than that of the conventional infant mouse test and comparable to that of recently described radioimmunoassays for ST.