Open AccessThe calculation of cellular parameters from the turbidity of yeast cultures may give rise to important errors
Author(s) -
Lagunas Rosario,
Moreno Eulalia
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00886.x
Subject(s) - turbidity , yeast , intracellular , dry weight , biological system , biology , volume (thermodynamics) , microbiology and biotechnology , chemistry , biochemistry , ecology , botany , physics , thermodynamics
It is shown that cellular parameters of the yeast cultures (e.g. intracellular volume, cellular dry weight, protein content, number of cells, and turbidity) are differently influenced by metabolic changes. Therefore, the cellular parameters change independently of each other. It is hence concluded that whenever quantitation is required, the values of these parameters should be measured independently and not calculated from the turbidity of the cultures or other parameters, as is often done.