Open AccessTransposon mutagenesis of the Pseudomonas aeruginosa PAO chromosome and the isolation of high frequency of recombination donors
Author(s) -
O'Hoy K.,
Krishnapillai V.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00880.x
Subject(s) - transposon mutagenesis , transposable element , plasmid , biology , genetics , mutagenesis , chromosome , sleeping beauty transposon system , mutant , pseudomonas aeruginosa , dna , gene , bacteria
The transposons Tn 5 and Tn 2521 have been loaded on to a temperature‐sensitive, replication‐defective mutant of the promiscuous IncP‐1 plasmid R68 and used for transposon mutagenesis of the Pseudomonas aeruginosa PAO chromosome. Tn 5 was found to be particularly useful for the generation of new auxotrophic mutations and Tn 2521 for the generation of a range of high frequency of recombination donors by plasmid integration into the chromosome. The new origins and directions of chromosome transfer mediated by this conditional plasmid replication system will greatly facilitate improved genetic mapping of PAO.