Open AccessProduction by non‐O1 Vibrio cholerae of hemolysin related to thermostable direct hemolysin of Vibrio parahaemolyticus
Author(s) -
Yoh Myonsun,
Honda Takeshi,
Miwatani Toshio
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00861.x
Subject(s) - hemolysin , vibrio parahaemolyticus , vibrio cholerae , microbiology and biotechnology , vibrionaceae , biology , polyacrylamide gel electrophoresis , immunoelectrophoresis , chemistry , bacteria , enzyme , antigen , biochemistry , virulence , gene , genetics
The production by non‐O1 Vibrio cholerae of a hemolysin immunologically related to the thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus was demonstrated by enzyme‐linked immunosorbent assay and a double gel diffusion test. Although results by the double gel diffusion test suggested the immunological identities of TDH and the TDH‐related hemolysin of non‐O1 V. cholerae , conventional polyacrylamide gel disc electrophoresis and immunoelectrophoresis suggested some differences between the two, at least with respect to charge. The TDH‐related hemolysin of non‐O1 V. cholerae was also shown to differ from the hemolysin of non‐O1 V. cholerae reported previously.