Open AccessNumerical analysis of Leptospira DNA‐restriction endonuclease patterns
Author(s) -
Hookey J.V.,
Waitkins Sheena A.,
Jackman P.J.H.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00858.x
Subject(s) - upgma , restriction enzyme , dendrogram , absorbance , similarity (geometry) , biology , restriction fragment , restriction fragment length polymorphism , endonuclease , genetics , strain (injury) , dna , microbiology and biotechnology , chromatography , chemistry , polymerase chain reaction , genotype , computer science , gene , anatomy , artificial intelligence , population , demography , sociology , genetic diversity , image (mathematics)
Fifteen test strains, representing 5 leptospire serogroups were studied by DNA‐restriction endonuclease ( Eco RI) analysis. The resulting patterns for each strain were scanned by a densitometer and the absorbance values were recorded along the pattern at regular intervals. The absorbance profiles were compared using the correlation coefficient and the similarity matrix was clustered by the UPGMA algorithm. The results were displayed as a simplified dendrogram. The numerical analysis of DNA‐restriction endonuclease patterns can be used successfully in the identification of Leptospira strains.