Open AccessMolecular cloning of a possible excretion protein of Vibrio cholerae
Author(s) -
Focareta Tony,
Manning Paul A.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00853.x
Subject(s) - vibrio cholerae , periplasmic space , escherichia coli , biology , transposable element , plasmid , microbiology and biotechnology , molecular cloning , cloning (programming) , gene , dna , structural gene , genetics , mutant , peptide sequence , bacteria , computer science , programming language
The gene for a Vibrio cholerae protein of about kDa (kilodalton) has been cloned and its location within the 1.9‐kb cloned DNA fragment determined by transposon insertion and deletion analyses. The proteins encoded within the various plasmids have been analyzed in Escherichia coli K‐12 minicells. The 25‐kDa protein when expressed in E. coli K‐12 allows the release of the periplasmic deoxyribonuclease. It is a minor protein suggesting that the release of DNase is not an artefact due to membrane damage. It is possible that this protein functions as part of an excretion system. Results with transposon Tn 1725 insertions suggest that it contains a termination site in one orientation and a promoter in the other.