Open AccessRelationship between carbon monoxide dehydrogenase and a small plasmid in Pseudomonas carboxydovorans
Author(s) -
Kwon M.O.,
Kim Y.M.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00852.x
Subject(s) - plasmid , carbon monoxide dehydrogenase , microbiology and biotechnology , biology , pseudomonas , enzyme , gene , carbon monoxide , chemistry , biochemistry , bacteria , genetics , catalysis
Abstract Isolation of plasmid DNA followed by plasmid curing was carried out to examine the relationship of plasmid to carbon monoxide dehydrogenase (CO‐DH) production in carboxydobacteria. A small plasmid of almost identical size (1.52−1.76 × 10 6 ) was present in Pseudomonas carboxydovorans, Azotobacter sp.1, and Azomonas sp.2. Azomonas sp.1 contained two kinds of plasmids (1.5 × 10 6 and 2.47 × 10 6 ). No plasmids were found in Pseudomonas carboxydohydrogena , JC1, and HY1. A plasmid‐cured clone of P. carboxydovorans was obtained by growing the cells at 37°C. The cured cell was able to grow CO autotrophically on solid, but not in liquid, medium. CO‐DH of the cured cell was active and consisted of three subunits similar to those found in the wild‐type enzyme, with the exception that the β subunit of the enzyme was larger than that of the wild‐type enzyme. These results suggest that the small plasmids do not carry genes encoding CO‐DH but may have gene(s) for processing the β subunit of the enzyme.