Open AccessCloning of ten distinct DNA fragments of Clostridium thermocellum coding for cellulases
Author(s) -
Millet Jacqueline,
Pétré Dominique,
Béguin Pierre,
Raynaud Odette,
Aubert JeanPaul
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00850.x
Subject(s) - clostridium thermocellum , cellulase , cloning (programming) , escherichia coli , dna , molecular cloning , gene , biochemistry , hydrolysis , chemistry , biology , microbiology and biotechnology , gene expression , computer science , programming language
Ten distinct Eco RI fragments of Clostridum thermocellum DNA have been cloned in Escherichia coli and shown to express enzymatic activities related to cellulose degradation. Two of the cloned fragments appeared to carry the previously characterized celA and celB genes, which code for the endoglucanases (EG) A and B. Five other cloned fragments code for hitherto unidentified EGs, which can be detected by the Congo red test for hydrolysis of carboxymethylcellulose (CMC). In addition, three separate clones hydrolyzed methylumbelliferyl‐β‐cellobioside (MUC) but not CMC, hinting that they may express three different cellobiohydrolase genes.