Open AccessA rapid procedure to detect the autolysin phenotype in Streptococcus pneumoniae
Author(s) -
García Ernesto,
Ronda Concepción,
García JoséLuis,
López Rubens
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00839.x
Subject(s) - autolysin , streptococcus pneumoniae , microbiology and biotechnology , transformation (genetics) , mutant , mutation , phenotype , biology , strain (injury) , transposable element , gene , genetics , antibiotics , anatomy
A simple and rapid procedure to detect autolysin‐defective mutants of Streptococcus pneumoniae has been developed. The autolysin gene ( lyt ) can be introduced into the appropriate receptor strain by genetic transformation and the transformants are readily detected on the surface of semisynthetic medium (C medium) plates by using a membrane filter. A pneumococcal autolysin mutation ( lyt ‐4) behaved as a low‐efficiency marker in genetic transformation.