Open AccessHypertonicity‐induced phosphorylation of proteins in Dictyostelium discoideum
Author(s) -
Okamoto Koji
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00826.x
Subject(s) - dictyostelium discoideum , cycloheximide , phosphorylation , biochemistry , biology , microbiology and biotechnology , dictyostelium , heat shock protein , protein biosynthesis , gene
By exposing the cells of Dictyostelium discoideum to a high concentration (120 mM) of KCl, several species of proteins (188 kilodalton (kDa), 95 kDa, and 71 kDa) are specifically phosphory‐lated. This phosphorylation is induced irrespective of the time of starvation of cells by KCl, but not by cAMP, and inhibited by cycloheximide. The 32 P‐labeled phosphoryl groups of 95‐ and 71‐kDa proteins disappear by chasing during the subsequent differentiation step in a liquid shake culture. The majority of the 188‐ and 95‐kDa proteins exist in the plasma membrane fraction.