Open AccessComposition of Caulobacter crescentus murein synthesized in the presence of glycine
Author(s) -
Markiewicz Zdzislaw,
Kraus Werner
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00824.x
Subject(s) - caulobacter crescentus , glycine , biochemistry , amino acid , alanine , peptidoglycan , peptide , biology , chemistry , in vivo , in vitro , cell wall , gene , microbiology and biotechnology , bacterial protein
Glycine added to the growth medium of Caulobacter crescentus was found to substitute Cterminal alanine in the peptide side chains of the murein of this species. Murein synthesized in vivo and in vitro in the presence of glycerine was poorly crosslinked as was new murein formed in the presence of the amino acid. The reduced cross‐linkage seems to be due to the effect of glycine on the formation of trimeric muropeptides as revealed by high‐performance liquid chromatography (HPLC) muropeptide analysis of murein formed in the presence and absence of the amino acid.