Open AccessPhosphorylation of hexoses in Streptomyces aureofaciens : Evidence that the phosphoenolpyruvate: Sugar phosphotransferase system is not operative
Author(s) -
Novotná Jana,
Hoš?álek Zdeněk
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00818.x
Subject(s) - phosphoenolpyruvate carboxykinase , pep group translocation , fructose , streptomyces aureofaciens , biochemistry , phosphotransferase , sugar , chemistry , phosphorylation , phosphofructokinase , biology , glycolysis , metabolism , streptomyces , enzyme , bacteria , genetics
Sugar phosphates are formed in cell‐free extracts of Streptomyces aureofaciens RIA57 from glucose or fructose in the presence of phosphoenolpyruvate. In contrast to the phosphorylation by adenosine 5′‐triphosphate the kinetics of formation of glucose 6‐phosphate via phosphoenolpyruvate (PEP) is nonlinear. The product of fructose phosphorylation (only fructose 6‐phosphate was determined by paper chromatography) and the absence of 1‐phosphofructokinase indicate that fructose metabolism in S. aureofaciens does not proceed via the phosphoenolpyruvate:sugar phosphotransferase system (PTS).