Open AccessThe applicability of redox‐indicator dyes in strongly reduced media; their effect on the human fecal flora
Author(s) -
Ruselervan Embden J.G.H.,
BothPatoir H.C.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00817.x
Subject(s) - dithiothreitol , redox , cresyl violet , chemistry , feces , human feces , anaerobic exercise , biochemistry , inorganic chemistry , microbiology and biotechnology , biology , staining , physiology , genetics , enzyme
Cresyl violet acetate was found to be an appropriate redox‐indicator dye in anaerobic culture media with low redox potentials. Low redox potentials ( E ′ h −250 to −300 mV) in media were obtained by addition of dithiothreitol (DTT). DTT and cresyl violet acetate in media did not influence the total number of anaerobes cultured from human feces.