Open AccessCloning and genetic organization of the gene cluster encoding F7 1 fimbriae of a uropathogenic Escherichia coli and comparison with the F7 2 gene cluster
Author(s) -
Die Irma,
Spierings Gonnie,
Megen Ingrid,
Zuidweg Elly,
Hoekstra Wiel,
Bergmans Hans
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00815.x
Subject(s) - gene cluster , cosmid , complementation , biology , gene , escherichia coli , plasmid , microbiology and biotechnology , cloning (programming) , genetics , mutant , coding region , computer science , programming language
The gene cluster coding for expression of F7 1 fimbriae of the uropathogenic Escherichia coli strain AD110 has been cloned by a cosmid‐cloning procedure. A positive clone was further subcloned to a plasmid of 17.5 kilobases (kb), pPIL110‐75. Analysis of pPIL110‐75 showed that at least six genes are present encoding proteins with apparent M r s of 75 000, 36 000, 23 000, 20 000, 17 000 and 14 000. The 20‐kDa protein, encoding the F7 1 fimbrial subunit is dispensable for expression of the MRHA phenotype. Complementation experiments of mutants in the F7 2 gene cluster by gene products of the F7 1 gene cluster show that the two gene clusters are related.