Open AccessSuppression by temperature of sporulation and of exocellular metalloproteinase synthesis in Bacillus megaterium
Author(s) -
Kučerová Helena,
Chaloupka Jiří
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00808.x
Subject(s) - sporangium , spore , incubation , bacillus megaterium , metalloproteinase , enzyme , biochemistry , chemistry , messenger rna , biology , microbiology and biotechnology , bacteria , gene , genetics
Temperatures slightly supraoptimal for growth (above 40°C) delayed or inhibited the development of sporangia as well as the synthesis of metalloproteinase. The inhibition of sporulation was reversible and most of the cells sporulated and excreted proteinase when transferred to 35°C. The ability to synthesize the enzyme in a sporulation medium was maximal between 0.5–1.0 h of incubation at 35°C, between 0.5–2.0 h at 43.5°C and even later at 45°C. Proteinase formation was suppressed by temperature at the level of mRNA transcription.