Open AccessChanges in Saccharomyces cerevisiae ascospore proteins during early germination
Author(s) -
West Thomas P.,
Armstrong Robert L.,
Magee Paul T.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00801.x
Subject(s) - ascospore , yeast , saccharomyces cerevisiae , biology , germination , ascomycota , polyacrylamide gel electrophoresis , gel electrophoresis , methionine , biochemistry , botany , spore , gene , amino acid , enzyme
Proteins present in Saccharomyces cerevisiae ascospores and in germinating ascospores were compared using two‐dimensional polyacrylamide gel electrophoresis. Yeast ascospores incorporated labeled methionine efficiently facilitating the electrophoretic analysis. Proteins synthesized in the yeast ascospores differed significantly from those proteins found 15 min after the initiation of germination in the ascospores. An immediate transition from ascospore proteins to proteins required for ascospore germination appears likely.