Open AccessReconstitution by a butyrolactone autoregulator of the parental protein pattern in an asporogenous mutant of Streptomyces griseus
Author(s) -
Gräfe U.,
Sarfert Eva
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00800.x
Subject(s) - streptomyces griseus , mutant , biology , strain (injury) , effector , gene , protein biosynthesis , biochemistry , proteolysis , microbiology and biotechnology , genetics , streptomyces , enzyme , bacteria , anatomy
The asporogenous anthracycline‐negative mutant 86 (Amy − Ant − ) of Streptomyces griseus was distinguishable from the parental strain JA5142 by an altered composition of the mycelial proteins. Addition of trans ‐2‐(6′‐methylheptanol‐1′‐yl)‐3‐hydroxymethyl‐4‐butanolide (factor I) to submerged cultures caused the adaptation of its protein pattern to that of the parental strain, concomitant with the restoration of anthracycline biosynthesis and morphological changes. In addition to the other effects of factor I on metabolic activity, the observed changes could be accounted for, at least partly, by alterations in intramycelial proteolysis. On the other hand, in the presence of factor I, some protein concentrations remained unaffected, supporting the view that a pleiotropic gene is involved in the synthesis of the autoregulatory effector by the parental strain S. griseus JA5142.