Open AccessNovel media for detection of microbial producers of cellulase and xylanase
Author(s) -
Farkaš V.,
Lišková Mária,
Biely P.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00779.x
Subject(s) - cellulase , xylanase , xylan , agar , chemistry , food science , microorganism , brilliant green , polysaccharide , agar plate , nutrient agar , cellulose , biochemistry , bacteria , chromatography , enzyme , biology , organic chemistry , genetics
Agar nutrient media containing 0.2% soluble hydroxyethylcellulose covalently dyed with Ostazin Brilliant Red H‐3B or soluble beechwood 4‐O‐methyl‐ d ‐glucurono‐ d ‐xylan dyed with Remazol Brilliant Blue R were used for sensitive detection of microorganisms producing and secreting into the surrounding medium endo‐1,4‐β‐glucanase and/or endo‐1,4‐β‐xylanase. Pale clearing zones formed around the colonies grown on such media indicated the production of corresponding polysaccharide‐hydrolases.