Open AccessF‐plasmid gene srnB + complements the function of the S gene of phage lambda
Author(s) -
Ohnishi Yoshinari,
Ono Tsuneko
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00690.x
Subject(s) - lysogen , plasmid , lytic cycle , lambda phage , gene , lysogenic cycle , pbr322 , microbiology and biotechnology , mutant , bacteriophage , biology , strain (injury) , genetics , escherichia coli , virus , anatomy
The srnB + gene located on the F plasmid was assayed for its capacity to facilitate the release from infected cells of phage λ lacking the usual lytic activity. The srnB + plasmid pOY54, carrying the 1.4–2.5F fragment in the Eco RI‐ Bam HI fragment of pBR322, induced bacteriolysis and the release of progeny phage of the λcI 857 susS 7 lysogen in the presence of rifampin at 42°C. An srnB 1 mutant plasmid, pOY541, did not promote bacteriolysis. These results suggest that the srnB + gene of the F plasmid complements the function of the λ S gene in the nonpermissive host strain.