Open AccessA comparison of lectin‐binding activity in two strains of Rhizobium japonicum *
Author(s) -
Robert Françoise M.,
Schmidt E.L.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00683.x
Subject(s) - lectin , soybean agglutinin , hemagglutination , biology , rhizobium , biochemistry , mannan binding lectin , microbiology and biotechnology , agglutinin , antigen , gene , immunology
The possibility was investigated that the dominance of native Rhizobium japonicum serogroup 123 in the nodules of soybean may be correlated with a superior ability to bind soybean lectin. A hemagglutination inhibition assay was used to quantify the lectin‐binding activity of strains 110 and 123. Addition of root extract to defined medium considerably enhanced lectin‐binding per cell in both strains. Lectin‐binding activity per cell (higher at 20°C than at 25°C or 30°C) was also growth‐dependent. Inactivation of lectin‐binding polysaccharide, accumulated in the medium, occurred with aging of the culture. No evidence was found for superior lectin‐binding ability in strain 123.