Open AccessVersatile shuttle cloning vectors for the unicellular cyanobacterium Anacystis nidulans R2
Author(s) -
Lau Reginald H.,
Straus Neil A.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00677.x
Subject(s) - shuttle vector , cloning (programming) , multiple cloning site , transformation (genetics) , plasmid , cloning vector , amp resistance , kanamycin , biology , selectable marker , escherichia coli , molecular cloning , vector (molecular biology) , aspergillus nidulans , genetics , reporter gene , origin of replication , gene , recombinant dna , gene expression , computer science , programming language , mutant
3 new shuttle cloning vectors for gene transfer into Escherichia coli and Anacystis nidulans have been constructed by utilizing the cyanobacterial origin of replication of the small plasmid pANS from A. nidulans . 2 of these new vectors, pXB7 (pDPL13 derivative) and pECAN8 (pUC8 derivative), convey ampicillin resistance, and transform A. nidulans with relatively high frequencies. Vector pXB7 has 10 unique cloning sites; pECAN8 contains 4 cloning sites within the lacZ gene permitting rapid detection of DNA inserts in the presence of Xgal. The third vector, pKBX, has a lower transformation frequency but adds kanamycin resistance as a selectable gene for shuttle vectors of cyanobacteria.