Open AccessThe association on SDS‐polyacrylamide gels of lipopolysaccharide and outer membrane proteins of Pseudomonas aeruginosa as revealed by monoclonal antibodies and Western blotting
Author(s) -
Poxton Ian R.,
Bell Graham T.,
Barclay G.Robin
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00676.x
Subject(s) - monoclonal antibody , polyacrylamide gel electrophoresis , blot , bacterial outer membrane , gel electrophoresis , lipopolysaccharide , microbiology and biotechnology , membrane protein , chemistry , antibody , immunoprecipitation , biology , biochemistry , membrane , escherichia coli , gene , immunology , enzyme
Monoclonal antibodies raised against single serotype components of a Pseudomonas aeruginosa vaccine have been shown to bind to the O antigen region of lipopolysaccharide (LPS). Outer membrane (OM) proteins, prepared by detergent treatment of envelope fractions and by EDTA/sonication treatment of whole cells, were separated on sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), electrophoretically transferred to nitrocellulose membrane and reacted with LPS‐specific monoclonal antibodies. The patterns produced revealed that many of the protein bands were in fact protein‐LPS complexes.