Open AccessRegulation of protoxin synthesis in Bacillus thuringiensis : conditional synthesis in a variant is suppressed by d ‐cycloserine
Author(s) -
Aronson A.I.,
Dunn P.E.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00674.x
Subject(s) - bacillus thuringiensis , cycloserine , plasmid , mutant , microbiology and biotechnology , biology , bacillus cereus , strain (injury) , gene , bacteria , antibiotics , biochemistry , genetics , anatomy
A variant of Bacillus thuringiensis subsp. kurstaki HD1 produced parasporal inclusions only at 25°C. At 32°C, there was no accumulation of transcripts of the plasmid‐encoded protoxin gene. This conditional phenotype was suppressed by a sub‐inhibitory concentration of d ‐cycloserine, in a d ‐cycloserine‐resistant mutant of the conditional strain, or by transfer of protoxin‐encoding plasmids to d ‐cycloserine‐resistant Bacillus cereus . Neither resistance to streptomycin nor sublethal concentrations of other antibiotics interfering with peptidoglycan synthesis suppressed this phenotype. Specific metabolic properties of the host cell appear to be involved in the regulation of protoxin synthesis.