Open AccessAnalyses of ribosomal proteins from mycobacteria with particular reference to the β antigen
Author(s) -
Lönnroth Ivar,
Ridell Malin
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00668.x
Subject(s) - mycobacterium phlei , ribosome , ribosomal protein , ribosomal rna , protein subunit , escherichia coli , polyacrylamide gel electrophoresis , biology , gel electrophoresis , 50s , antigen , mycobacterium , microbiology and biotechnology , biochemistry , chemistry , bacteria , genetics , rna , enzyme , gene
The proteins of the ribosomal subunits of Mycobacterium phlei were analysed by sodium dodecylsulphate (SDS)‐polyacrylamide gel electrophoresis (PAGE) and by 2‐dimensional PAGE. These techniques revealed that both the size and the charge of the mycobacterial ribosomal proteins were quite different from those of Escherichia coli . The divergent chemical properties of the mycobacterial ribosomes might be related to other exceptional properties of mycobacteria, e.g., their slow growth. An antigen designated β was furthermore revealed in both 30S and 50S subunits of M. phlei and Mycobacterium bovis BCG. 2 Proteins from each subunit migrated in a similar way in SDS‐PAGE, being thus prime candidates as carriers of β‐activity.