Open AccessInfluence of the composition of the fusion medium on the yield of electrofused yeast hybrids
Author(s) -
Schnettler R.,
Zimmermann U.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb00666.x
Subject(s) - electrofusion , hybrid , yeast , yield (engineering) , sorbitol , fusion , bivalent (engine) , chemistry , protoplast , cell fusion , isotonic , biochemistry , biology , botany , materials science , organic chemistry , linguistics , philosophy , metallurgy , medicine , cell , metal
Electrofusion between cells of yeast strains with different genetic markers in isotonic sorbitol solutions leads to high yields of hybrids when 0.1 mM Ca 2+ and 0.5 mM Mg 2+ salts are aded. On average, 1000–2000 hybrids are obtained when electrofusion is performed (in a helical chamber) compared to a yield of about 40–120 in the absence of these bivalent cations. A further increase in yield can be achieved by the addition of 1 mg/ml albumin, which results in up to 4000 hybrids per experimental run. The entire fusion process leads to very reproducible results in the presence of these substances.