Open AccessLigninolytic enzyme production by Phanerochaete chrysosporium under conditions of nitrogen sufficiency
Author(s) -
Buswell John A.,
Mollet Bruno,
Odier Etienne
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01475.x
Subject(s) - phanerochaete , chrysosporium , glycerol , chemistry , nitrogen , enzyme , lignin , food science , hydrogen peroxide , enzyme assay , metabolite , biochemistry , organic chemistry
A lignin‐degrading enzyme has been detected in culture supernatants of Phanerochaete chrysosporium strain INA‐12 grown under non‐limiting nitrogen conditions. Highest levels of enzyme activity were observed when glycerol served as carbon source. Veratryl alcohol, a known secondary metabolite of P. chrysosporium , was also produced in high nitrogen/glycerol cultures of strain INA‐12 and closely followed the development of the ‘ligninase’ activity. Evolution of 14 CO 2 from 14 C‐ring‐DHP was readily observed when a hydrogen peroxide‐generating system was added to 5‐day‐old high nitrogen/glycerol cultures which contained high amounts of enzyme.