Open AccessMolecular cloning and physical mapping of the hyd gene of Escherichia coli K‐12
Author(s) -
Karube Isao,
Tomiyama Masamitsu,
Kikuchi Akihiko
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01448.x
Subject(s) - ecori , plasmid , escherichia coli , operon , restriction enzyme , molecular cloning , psti , hybrid plasmid , restriction map , biology , gene , pbr322 , bamhi , microbiology and biotechnology , mutant , hydrogenase , recombinant dna , cloning (programming) , genetics , bacteria , gene expression , computer science , programming language
The hyd (hydrogenase activity) gene of Escherichia coli K‐12 was cloned in a recombinant plasmid designated pEH1 and a restriction endonuclease map for the enzymes Eco RI, Bam HI, Sal I, and Pst I was constructed. This gene was shown to be located at about 6 kilobase pairs (kb) away from the srl operon. Plasmid pEH1 and its derivatives, pEH2 and pEH3 restored hydrogenase activity and fermentative production of hydrogen from glucose in hyd mutant to the wild‐type level.