Open AccessPhysical map of the bacteriophage L ( Salmonella typhimurium )
Author(s) -
Karlovský Petr,
Soška Jiší,
Reich Josef,
Kuhrová Viera
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01386.x
Subject(s) - ecori , bamhi , plasmid , psti , restriction enzyme , restriction map , bacteriophage , salmonella , biology , genetics , genome , recombinant dna , microbiology and biotechnology , gene , escherichia coli , bacteria
A circular restriction map of the genome of the phage L ( Salmonella typhimurium ) has been constructed with five restriction endonucleases, Eca I, Eco RI, Bam HI, Bgl I, and Pst I. The Eco RI fragments of phage‐L DNA were cloned into pACYC184, and the resulting recombinant plasmids pL1, pL2,…,pL7 were introduced into Salmonella typhimurium . The genes present on the fragments cloned were identified by the marker rescue experiments with the L‐phage amber mutants. A physical gene map of the L genome obtained in this way was compared with that of P22.