Open AccessCharacterization of a plasmid carrying the Escherichia coli K‐12 entD, fepA, fes , and entF genes
Author(s) -
Coderre Peter E.,
Earhart Charles F.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01385.x
Subject(s) - subcloning , plasmid , escherichia coli , complementation , gene , biology , gene cluster , chemistry , genetics , microbiology and biotechnology , phenotype
The plasmid pMS101 carries Escherichia coli K‐12 genes ( entD, fes, entF ) essential for enterochelin‐mediated iron transport [Laird, A.J. and Young, I.G., Gene 11 (1980) 359–366]. We have further characterized pMS101 and shown that it also contains the gene ( fepA ) for the 81 000 Da outer membrane ferrienterochelin receptor. Subcloning experiments in conjunction with complementation and maxicell studies demonstrated the gene order to be entD fepA fes entF . The entF ‐ and fes ‐encoded polypeptides were found to be 115 000 and 42 000‐Da soluble proteins, respectively. Plasmid‐borne enterochelin cluster genes were overexpressed in iron‐deficient conditions and their products were undetectable under iron‐replete conditions.