Open AccessConjugal transfer of pAMβ1 in Bacillus sphaericus 1593
Author(s) -
Orzech Karen A.,
Burke William F.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01382.x
Subject(s) - bacillus sphaericus , plasmid , bacillaceae , microbiology and biotechnology , biology , bacillales , bacteria , biochemistry , genetics , bacillus subtilis , gene
The plasmid pAMβ1 has been successfully transferred from Streptococcus faecalis to Bacillus sphaericus by the method of filter mating. Subsequently, transfer of this conjugative plasmid has also been demonstrated between derivatives of B. sphaericus 1593, though at reduced frequencies. The transfer process was determined to be unaffected by either endogenous restriction enzyme activity of the recipient or the presence of exogenous DNase.