Open AccessAn unexpected junction in a thrA‐lacZ hybrid gene of Escherichia coli constructed in vivo using Mu and lambda
Author(s) -
Parsot Claude,
Cossart Pascale,
Margarita Danielle,
SaintGirons Isabella
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01377.x
Subject(s) - gene , nucleotide , genetics , dna , biology , gene product , nucleic acid sequence , fusion gene , microbiology and biotechnology , gene expression
The DNA sequence of the fusion joint of a hybrid gene whose product carries both aspartokinase and β‐galactosidase activities has been determined. Four supplementary nucleotides were found which do not belong to the original genes. Those nucleotides could be attributed to the S‐end of Mu, but they are not in the proper orientation considering the technique of gene fusion used, which involved Mu and λ. This result may bear on the specificity and mechanism of Mu insertion, as well as on the nature of Mu induced DNA rearrangements.