Open AccessProtein synthesis during outgrowth of Bacillus subtilis spores. A two‐dimensional gel electrophoresis study
Author(s) -
Hecker Michael,
Wachlin Gerhild,
Dunger AnneMarie,
Mach Friedrich
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01375.x
Subject(s) - bacillus subtilis , spore , gel electrophoresis , biology , protein biosynthesis , gene expression , two dimensional gel electrophoresis , gene , polyacrylamide gel electrophoresis , electrophoresis , microbiology and biotechnology , biochemistry , bacteria , proteomics , genetics , enzyme
Two‐dimensional gel electrophoresis was used to study protein synthesis during various stages of outgrowth of Bacillus subtilis spores. Protein synthesis throughout outgrowth is a highly ordered process. Different classes of proteins were distinguished by the time of onset and duration of their synthesis. During all stages of outgrowth a great number of vegetative proteins is synthesized. A second group of vegetative proteins is detected which are not produced during early outgrowth but during middle and later stages only. A third group of proteins is synthesized during early outgrowth but not in vegetative cells. Mechanisms for the regulation of expression of vegetative genes during outgrowth are discussed.