Open AccessAcetate catabolism by Methanosarcina barkeri : Hydrogen‐dependent methane production from acetate by a soluble cell protein fraction
Author(s) -
Krzycki J.A.,
Zeikus J.G.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01369.x
Subject(s) - methanosarcina barkeri , methanogenesis , chemistry , biochemistry , carbon monoxide dehydrogenase , methane , methanosarcina , carbon dioxide , organic chemistry , catalysis , carbon monoxide
Cell extracts prepared from Methanosarcina barkeri converted acetate into methane and carbon dioxide under a hydrogen atmosphere. Methanogenesis by cell extracts required acetate and ATP and, the in vitro rate was 5 to 10% of the rate of methanogenesis observed during exponential growth of cells on acetate. Methane and carbon dioxide produced by cell extracts originated predominantly from the methyl and carboxyl groups of acetate, respectively, in a manner consistent with that observed in whole cells. Acetate degradation activity was detected in the soluble (150000 × g supernatant) fraction and not in the membrane fraction. These results are discussed in relation to a proposed model for ATP generation from acetate that involves both membrane‐bound and soluble enzymatic components such as CO dehydrogenase.