Open AccessPurification and some properties of an extracellular halophilic 5′‐nucleotidase from a moderate halophile, Micrococcus varians subsp. halophilus
Author(s) -
Onishi Hiroshi,
Kobayashi Takekazu,
Kamekura Masahiro
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01324.x
Subject(s) - halophile , micrococcus , extracellular , ethanol precipitation , biochemistry , enzyme , sarcina , chemistry , chromatography , biology , bacteria , ethanol , genetics
An extracellular 5′‐nucleotidase produced by a moderate halophile, Micrococcus varians subsp. halophilus was partially purified from the culture filtrate by ethanol precipitation and Sepharose 4B hydrophobic chromatography. The 5′‐nucleotidase was a novel halophilic enzyme, requiring 2 M NaCl or 2.5 M KCl and 0.1 mM Co 2+ or 0.1 mM Mn 2+ for maximal activity.