Open AccessNMR spectrometric assay for determining enzymatic hydrolysis of β‐lactam antibiotics with bacteria in aqueous solution
Author(s) -
O'hara Koji,
Shiomi Yoko,
Kono Megumi
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01322.x
Subject(s) - chemistry , bacteria , enzymatic hydrolysis , hydrolysis , aqueous solution , chromatography , nuclear magnetic resonance spectroscopy , proton nmr , antibiotics , enzyme , biochemistry , organic chemistry , biology , genetics
An application of a nuclear magnetic resonance (NMR) spectrometer for the measurement of β‐lactamase activity in clinical material containing bacteria is presented. By means of proton ( 1 H)‐NMR, it was easy to measure quantitatively β‐lactamase activity in human bacteriuria, without performing any such pretreatment as isolation of bacteria or extraction of crude enzymes and without preparing special reagents for the detection. This is the first report on the application of 1 H‐NMR analysis of structural changes for determining hydrolysis of β‐lactam antibiotics with β‐lactamase‐producing bacteria in aqueous solution.