Open AccessThe number of anaerobically regulated genes in Escherichia coli
Author(s) -
Clark David P.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01314.x
Subject(s) - escherichia coli , gene , amino acid , biology , auxotrophy , lac operon , biochemistry , enterobacteriaceae , genetics , chemistry
A bank of 5000 random gene fusions to lacZ was screened for amino acid auxotrophs and for gene fusions in which β‐galactosidase was only expressed anaerobically. Since the number of genes involved in the synthesis of amino acids is known, estimation of the number of anaerobically regulated genes is possible. This estimate that Escherichia coli contains about 50 anaerobically induced genes, is somewhat larger but of the same order as the number of anaerobically induced polypeptides seen on the 2‐D gels of Smith and Neidhardt.