Open AccessOuter membrane antigens of mucoid Pseudomonas aeruginosa isolated directly from the sputum of a cystic fibrosis patient
Author(s) -
Anwar Hosmin,
Brown Michael R.W.,
Day Alan,
Weller Peter H.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01311.x
Subject(s) - pseudomonas aeruginosa , antigenicity , sputum , microbiology and biotechnology , cystic fibrosis , tobramycin , piperacillin , antigen , bacterial outer membrane , antibiotics , biology , chemistry , bacteria , medicine , immunology , gentamicin , pathology , tuberculosis , biochemistry , escherichia coli , genetics , gene
The antigenicity of the outer membrane components of mucoid Pseudomonas aeruginosa directly isolated from the sputum of a cystic fibrosis patient and those of the same isolate cultivated under iron‐depleted conditions in the presence of sub‐in‐hibitory concentrations of piperacillin and/or tobramycin was investigated by immunoblotting using the patient's own serum. The results indicated that iron‐regulated membrane proteins as well as other major outer membrane proteins were antigenic and recognised by the patient's serum. The antibiotics used profoundly influenced the surface antigen pattern.