Open AccessA simple method for directly obtaining thermosensitive mutants of non‐essential genes: selection of cya ts and crp ts mutants in Escherichia coli K‐12
Author(s) -
Daniel Jacques
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01307.x
Subject(s) - mutant , selection (genetic algorithm) , gene , wild type , allele , biology , phenotype , genetics , computer science , artificial intelligence
We have directly selected thermosensitive alleles of cya and crp genes from the wild‐type, and present preliminary characterization of these mutants. The selection procedure is based on prior growth of a mutagenized wild‐type culture in a medium that counterselects, at low temperature, non‐conditional relative to thermosensitive mutants, followed by routine selection of mutants at high temperature. This method should be applicable to various genetic systems.